A tandem dye consists of a This dye has been developed exclusively by BD Biosciences as a brighter alternative to Alexa Fluor® 700. For Research Use Only. This optimal wavelength is called the excitation peak. However, APC tends to be brighter while Alexa Fluor® 647 is more optimal for intracellular applications. BV421 is a polymer-based dye excited by the violet laser and is one of the brightest fluorochromes offered by BD Biosciences. VioBright FITC is excited at 496 nm with a peak emission at 522 nm. APC-H7 is an APC-cyanine tandem fluorochrome, which uses an analog of Cy7 and has similar spectral properties to APC-Cy7. BD Horizon™ BUV661 is a tandem fluorochrome that combines BD Horizon BUV395 and an acceptor dye with an emission max at 657 nm. Due to similar excitation and emission properties, BB515, FITC, and Alexa Fluor® 488 cannot be used simultaneously. All products listed are labeled with some regulatory status as per indicated below: “RUO” or “For Research Use Only”: not intended for diagnostic or therapeutic procedures. The Cy5 molecule has been shown to exhibit nonspecific binding to Fc receptors, which is most apparent on monocyte populations. Search Due to the excitation of the BV421 donor by the 355 nm (UV) laser line, there may be spillover into the BUV737 detector off of the UV laser. The excitation and emission maxima are nearly identical to those of APC, albeit APC tends to be brighter. BD Horizon™ V500 is a novel organic dye excited by the violet laser. Peak excitation and emission wavelengths often vary depending on the environment in which the probe finds itself. Due to the excitation of the acceptor dye by the 561nm (Yellow-Green) laser line, there may be significant spillover into the PE-CF594 detector from the Yellow-Green laser. Here are some hints and tips on how to handle tandem dyes: Always protect tandem dyes from light as they are highly susceptible to photobleaching. This fluorochrome exhibits uncommon photostability, making it an ideal choice for use in fluorescence microscopy. Peridinin-Chlorophyll-Protein (PerCP) is a 35.5 kDa fluorescent peridinin-chlorophyll-protein complex isolated from dinoflagellates. BUV805 is designed for instruments equipped with a 355nm UV laser and a 820/60 filter. Alexa Fluor® 647 conjugates are highly photostable and remain fluorescent over a broad pH range. The carbocyanine-based dye exhibits far-red fluorescence with excitation at 642 nm and emission at 661 nm. BD Horizon V500 cannot be used simultaneously with AmCyan or Pacific Orange™. BB700 is a dye that was exclusively developed by BD Biosciences as brighter alternative to PerCP-Cy5.5. … PE-Cy™7 is a tandem fluorochrome that combines PE and a cyanine dye. This dye offers a very bright choice for the violet laser. R718 enables the resolution of low expression markers without having to switch to a tandem dye (APC-R700), which can introduce residual emission and resulting SOV/spread into the APC channel. Due to nearly identical excitation and emission properties but different spillover characteristics, BD Horizon BV421, Pacific Blue™, and BD Horizon V450 cannot be used simultaneously. This site uses cookies. FITC is relatively dim and should be reserved for highly expressed markers whenever possible. Excitation-Emission Matrix (EEM) is used with fluorescence spectroscopy for identification and characterization in mixture analysis. Within groups, roughly in order of excitation wavelength (families excepted). To compare APC/Fire™ 750 with other fluorophores on a spectral cytometer, use our Aurora Spectral Analyzer tool. Its basic form – Fluorescein – has a molar mass of 332 g/mol and is often used as a fluorescent tracer. This makes PE one of the brightest fluorochromes for flow cytometry applications, but its photobleaching properties make it unsuitable for fluorescence microscopy. APC has 6 phycocyanobilin chromophores per molecule, which are similar in structure to phycoerythrobilin, the chromophore in R-PE. Emission ; BP 385/30 BP 469/38 BP 555/30 BP 631/33 … To compare APC/Fire™ 750 with other fluorophores on a spectral cytometer, use our Aurora Spectral Analyzer tool. APC has six phycocyanobilin chromophores per molecule, which makes it a very bright fluorochrome that is highly suitable for flow cytometry applications. Not for resale. Therefore, Alexa Fluor® 647 is most commonly visualized with a confocal microscope equipped with an appropriate laser for excitation … BD Horizon™ BUV805 is a tandem fluorochrome that combines BD Horizon BUV395 and an acceptor dye with an emission max at 797 nm. Based on my perfunctory understanding of spectral overlap and compensation, this seems to make sense (eg. APC emits fluorescence at 660 nm. 300 0 20 40 60 80 350 400 640 nm Excitation and Emission Spectra of APC/Fire™ 750 . APC (Ex max 650 nm/Em max 660 nm), Allophycocyanin (APC), is an accessory photosynthetic pigment found in blue-green algae. Figure 1: Tandem dyes A. Diagrammatic representation of transfer of energy between donor and acceptor molecules in a tandem dye PE-Cy7. Emission . Its basic form – Fluorescein – has a molar mass of 332 g/mol and is often used as a fluorescent tracer. AmCyan is a 108-kDa protein derived from Anemonia majano. Fixed cells should be analyzed within 4 hours of fixation in paraformaldehyde or transferred to a paraformaldehyde-free buffer for overnight storage. Alexa Fluor® 700 is a far-red dye that can be excited with a 633–640-nm laser. This may include cookies from third party websites. APC tetramers are labeled with Streptavidin-Allophycocyanin (SA-APC), excitation 633–635 nm/emission 660–680 nm. For instruments equipped with a 561 nm Yellow-Green laser, a 585/15 nm filter is recommended for detection. However, because of its peak emission at 667 nm, Alexa Fluor® 647 cannot be seen well by eye, and it cannot be excited optimally with a mercury lamp. BV480 has less spillover into the BD Horizon™ BV605 detector and, in general, is brighter than BD Horizon BV510. Due to the close proximity of their emission spectra, there will be significant spillover from BV750 into BV786. BUV661 is designed for instruments equipped with a 355nm UV laser and a 670/25 filter. BD Horizon™ BUV563 is a tandem fluorochrome that combines BD Horizon BUV395 and an acceptor dye with an emission max at 560 nm. Allophycocyanin (APC), is an accessory photosynthetic pigment found in blue-green algae. Due to the excitation and emission characteristics of the acceptor dye, there will be spillover into the APC and Alexa Fluor® 700 detectors. BUV563 has been exclusively developed by BD Life Sciences. Figure 1 below illustrates the excitation and emission spectra of FITC under conditions of 488 nm excitation. Thermo Fisher Scientific. Exportable as image or link. Some APC-Cy7 conjugates show changes in their emission spectra with prolonged exposure to paraformaldehyde. R-HeNe APC-Cy5.5 633/695 LSRII/Aria/CantoII R-HeNe Alexa700 633/720 Aria R-HeNe APC-Cy7 (H7) 633/785 LSRII/Aria/CantoII Laser Fluorescent Protein Excitation/Emission nm Instrument UV BFP 360440 LSRII UV Sapphire 399/511 LSRII UV T-Sapphire 399/511 LSRII Violet eCFP 436/475 LSRII/Aria/CantoII Violet mCFP 433/476 LSRII/Aria/CantoII PE or APC) with an acceptor Like Alexa Fluor 633, APC is excited using the helium-neon red diode laser (633 nanometers) of the FACSCalibur and is detected using the FL4 detector. With an Ex Max of 436-nm and Em Max at 478-nm, BD Horizon BV480 can be excited by the violet laser and detected in the BD Horizon™ BV510 (525/40-nm) filter set. APC-Cy™7 is a tandem fluorochrome that combines APC and a cyanine dye (Cy7). It has a broad spectrum of excitation with the main peak at 482 nm, and a long Stokes shift to an emission … APC (Allophycocyanin) spectrum - APC (Allophycocyanin) is ... excitation and emission wavelengths using the interactive Spectrum Viewer - A web application for viewing and comparing spectra of various fluorescent compounds. The selection of a wideband, superwide band or narrow band filter cube will depend on the condition of the specimen and observation purpose. APC-H7 conjugates are typically 75% as bright as equivalent APC-Cy7 conjugates. Excitation/Emission Wavelengths and Filter Cube Suggestions Listed by Application The following tables contain data about various fluorescence excitation and emission properties of over 75 commonly used fluorochromes. PE-Cy7 is particularly sensitive to photo-induced degradation, resulting in loss of fluorescence and changes in fluorescence spillover. Due to the excitation and emission characteristics of the acceptor dye, there may be moderate spillover into the Alexa Fluor® 700 and PerCP-Cy5.5 detectors. Hence, the values to be taken as guidelines. BD Horizon™ BV480 is part of the BD Horizon Brilliant™ Violet family of dyes. If you do not wish to enable cookies please configure this here. APC-H7 conjugates provide greater stability in light and paraformaldehyde fixatives and have less spillover into the APC channel than APC-Cy7 conjugates. The emission of light from fluorescein, ranges from 490 nm to 630 nm, and the emission peak is approximately 520 nm. Excitation max (nm) 496: Emission max (nm) 785: Recommended for: Low density markers: Incompatible dyes: APC-Cy™7 (on instruments with collinear lasers) Comments: Compatible with green (532 nm) and yellow-green (561 nm) lasers; Tandem dye is sensitive to degradation. Excitation Emission PE-Cy 5® Laser line (488) Tandem (PE-Cy5 ® ) Excitation and Emission Spectral Profiles Energy Level Diagrams Fluorochromes have unique and characteristic spectra for absorption (excitation) and emission. In the US, call 1-800-4BIORAD (1-800-424-6723). Excitation Laser Line (nm) Fluorescence Channel Fluorochromes provided by BD Biosciences BD FACSArray™ bioanalyzer Green Diode 532 Yellow PE Far Red PerCP-Cy5.5 PE-Cy7 Red Diode 635 Red APC Alexa Fluor® 647 Infrared BD APC-H7 APC-Cy7 *BD FACSCalibur™ flow cytometry system Argon 488 FL1 Green FITC Alexa Fluor® 488 FL2 Yellow PE In addition alternative laser lines are becoming more affordable so dyes excited by 355 nm and 405 nm lasers are increasing the options for multiplexing. PE-CF594 is a brighter alternative to PE-Texas Red®, with improved spectral characteristics. For cell types that are not very autofluorescent, such as fresh cells, the improved separation seen with long-wavelength excitation … It has a 650 nm wavelength absorption maximum and a 660 nm fluorescence emission maximum. With an excitation peak of 457 nm and an emission peak of 491 nm, it can be used on violet laser–equipped flow cytometers in combination with BD Horizon BV421, BD Horizon V450, or Pacific Blue™, but not BD Horizon™ V500. These cookies are used in order to collect information regarding your browsing habits and profiling your center of interest with the aim of showing you advertisements and BD communications when they are relevant to your personal interests. Its fluorescent signal can be detected with a standard FITC filter, such as the 525/50 nm filter of the MACSQuant Instruments. Trademarks are the property of their respective owners. Excitation Emission PE-Cy 5® Laser line (488) Tandem (PE-Cy5 ® ) Excitation and Emission Spectral Profiles Energy Level Diagrams Fluorochromes have unique and characteristic spectra for absorption (excitation) and emission. Reagents conjugated to PE, PerCP, PerCP-Cy5.5, APC, and APC-Cy7 tandem fluorochrome can be used on leukocytes from these mouse strains. For Research Use Only. For cell types that exhibit high levels of autofluorescence, using a dye with a longer emission wavelength (e.g., APC, APC-Cy7) often provides an excellent signal-to-noise ratio. For more detailed information on specific cookies written for each of the categories below and their purpose please refer to our cookie policy. Conjugates are typically 10 times brighter than Pacific Blue™ conjugates and are often as bright as or brighter than PE conjugates. It is excited by laser lines at 594 and 633 nm, with an absorbance maximum at 650 nm and a fluorescence emission peak at 660 nm. BD Horizon™ BV750 is a tandem fluorochrome that combines BD Horizon BV421 and an acceptor dye with an emission max at 745 nm. APC-Cy™7 is a tandem fluorochrome that combines APC and a cyanine dye (Cy7). Due to nearly identical excitation and emission properties, but different spillover characteristics, APC and Alexa Fluor 647 cannot be used simultaneously. It has an excitation/emission peak at 495/517 nm and can be coupled to distinct antibodies with the help of its reactive isothiocyanate group, which is binding to amino, sulfhydryl, imidazoyl, tyrosyl or carbonyl groups on proteins. Extreme caution must be taken to avoid light exposure and prolonged exposure to paraformaldehyde fixative. When BD Horizon BUV563 is used with an instrument that does not have a 561 nm laser, a 560/40 nm filter may be more optimal. Therefore, Alexa Fluor® 647 is most commonly visualized with a confocal microscope equipped with an appropriate laser for excitation … We offer a broad range of APC products, including primary antibodies, secondary antibodies, streptavidin conjugates, and more. IIIIIIII Wavelength (nm) 350 400 450 500 550 600 650 700 For more information on Bio-Rad imaging products, contact your local Bio-Rad representative. Emission Excitation: 631 - 671 nm Emission: 640 - 680 nm. APC (Allophycocyanin)-Cy7 spectrum - APC (Allophycocyanin)-Cy7... excitation and emission wavelengths using the interactive Spectrum Viewer - A web application for viewing and comparing spectra of various fluorescent compounds. BV421 tetramers are labeled with Streptavidin-Brilliant Violet ™ 421 (SA-BV421), excitation maximum 405 nm/emission maximum 421 … Due to nearly identical excitation and emission properties but different spillover characteristics, FITC and Alexa Fluor® 488 cannot be used simultaneously. Allophycocyanin (APC) is an intensely bright phycobiliprotein isolated from red algae that exhibits far-red fluorescence with high quantum yields. Due to similar excitation and emission properties, BD Horizon BB700 and PerCP-Cy5.5 cannot be used simultaneously. This fluorochrome exhibits uncommon photostability, making it an ideal choice for use in fluorescence microscopy. Emission Max 785 nm. Simply click on the representative spectrum colors to view histograms that represent the absorption and emission spectra for each BD™ fluorochrome. In addition, RPE exhibits: We offer APC conjugated to a variety of primary antibodies, secondary antibodies, and streptavidin. BUV732 is designed for instruments equipped with a 355nm UV laser and a 740/35 filter. In practical applications, the sensitivity of APC conjugates is usually 5 to 10 times greater than that of the corresponding fluorescein conjugate. PerCP-Cy™5.5 is a tandem conjugate that combines PerCP with a cyanine dye. BD Horizon™ BUV737 is a tandem fluorochrome that combines BD Horizon BUV395 and an acceptor dye with an emission max at 732 nm. Exportable as image or link. BV750 is designed for instruments equipped with a 405nm Violet laser and a 750/30 filter. BD Horizon™ BV750 is a tandem fluorochrome that combines BD Horizon BV421 and an acceptor dye with an emission max at 745 nm. Due to the excitation of the acceptor dye by other laser lines, there may be spillover into channels detecting PE (eg, 575/26-nm filter) and PECF594 (eg, 610/20-nm filter). Thus, no changes in detection filters or the flow cytometer itself are required. When these two fluorophores are brought together by a biomolecular interaction, a portion of the energy captured by the Europium during excitation is released through fluorescence emission at 620 nm, while the remaining energy is transferred to the APC. BD Horizon™ Red 718 (R718) is a small molecule fluorochrome with an emission max near 718 nm. Due to the close proximity of their emission spectra, there will be significant spillover from BV750 into BV786. After excitation at 337 nm (from a nitrogen laser) the Eu 3+ –TBP complex emits at 580, 620, 650, and 700 nm, the highest peak being at 580 nm. the peak of the spectrum). PerCP is a component of the photosynthetic apparatus found in the dinoflagellate Glenodinium. The table below (Table 2) contains data on the peak excitation and emission wavelengths of over 75 selected fluorochromes along with suggested applications and excitation filter cube type. Due to similar excitation and emission properties, BV480, BV510 and V500 cannot be used simultaneously. The emission of light from fluorescein, ranges from 490 nm to 630 nm, and the emission peak is approximately 520 nm. After excitation at 337 nm (from a nitrogen laser) the Eu 3+ –TBP complex emits at 580, 620, 650, and 700 nm, the highest peak being at 580 nm. However, Alexa Fluor 647 is considered more optimal for intracellular applications. Dye APC (allophycocyanin) Excitation. Be sure to also look up the excitation and emission spectra for your dye of choice. Fluorochrome Selection Knowing the excitation and emission properties of fluorescent compounds makes it possible to select combinations of fluorochromes that will work together optimally on a specific flow cytometer with specific lasers. Conjugates are typically as bright or brighter than comparable reagents conjugated to Pacific Blue™. Because of its broad absorption range and the fact that its emission spectra are equivalent to APC, PE-Cy5 is not recommended for simultaneous use with APC. Super Bright Polymer Dyes are sold under license from Becton, Dickinson and Company. Due to the excitation and emission characteristics of the acceptor dye, there will be spillover into the APC and Alexa Fluor® 700 detectors. Emission Excitation: 631 - 671 nm Emission: 640 - 680 nm. Excitation-Emission Matrix (EEM) is used with fluorescence spectroscopy for identification and characterization in mixture analysis. BB515 is a dye that was exclusively developed by BD Biosciences as an additional bright dye for the blue laser. PE-Cy7 conjugated reagents are as bright as PE conjugates. Spectroscopy, Elemental & Isotope Analysis, Preclinical to Companion Diagnostic Development, Chromatography Columns, Resins, & Spin Filters, Secondary antibody conjugates and tandems, Isotype controls and tandem isotype controls, Design intracellular flow cytometry experiments in 5 time-saving steps, Beads for instrument controls and standards, Quench-resistance due to the protein backbone, Homogeneous structure with defined molecular weights. Recently, EEM has started to become an accepted method for determining the type, quality, and geographical origin of food products. “LUO” or “Laboratory Use Only”: general purpose laboratory reagents. with FITC/PE channels, the same excitation laser will cause emission that will be detected by both the FITC and PE detector, but different excitation lasers for APC… However, the closer the excitation wavelength is to 495 nm, the more fluorescence will be produced. The excitation spectrum is a range of light wavelengths that add energy to a fluorochrome, causing it to emit light in another range of wavelengths, the emission spectrum. It has a broad spectrum of excitation with the main peak at 482 nm, and a long Stokes shift to an emission … Dye APC (allophycocyanin) Excitation. Detail page for "Allophycocyanin(APC)" APC is a suitable energy acceptor because it has a high molar absorptivity in the range of Eu 3+ emission. BUV615 has been exclusively developed by BD Life Sciences. BV650 will have moderate spillover into the BD Horizon™ BV711 detector. Jackson ImmunoResearch offers the form found in Dinophyceae sp. Fluorophore Excitation and Emission Data 526SP 526 nm. Due to nearly identical excitation and emission properties but different spillover characteristics, APC and Alexa Fluor® 647 cannot be used simultaneously. Examples of tandem dyes include PE-Cy7, PE-Cy5.5, PE-Atto 594, and APC-Cy7. BV786 is a tandem fluorochrome of BD Horizon BV421 and an acceptor dye with an emission maximum at 786 nm. Cy™ is a trademark of GE Healthcare. → Energy transfer dye excited at ~488 nm with emission maximum at ~670 nm → Also known as PE-Cy®5 → Bright fluorescence → Low compensation against RPE → Significant emission in APC channel with 633 nm excitation → Measured in FL3 channel on … Excitation Max 650 nm. Excitation max (nm) 496: Emission max (nm) 785: Recommended for: Low density markers: Incompatible dyes: APC-Cy™7 (on instruments with collinear lasers) Comments: Compatible with green (532 nm) and yellow-green (561 nm) lasers; Tandem dye is sensitive to degradation. PerCP is a protein complex with a molecular weight of approximately 35 kDa. Due to the excitation of the acceptor dye by the green (532-nm) and yellow-green (561-nm) lasers, there will be significant spillover of the BD Horizon BV605 signal into the PE and BD Horizon PE-CF594 detectors off the green or yellow-green lasers. © 2020 BD. PerCP-Cy5.5 is not subject to photobeaching like PerCP and can be used with stream-in-air flow cytometers. Alexa Fluor® 488 exhibits extraordinary photostability, which makes it highly suitable for fluorescence microscopy. However, because of its peak emission at 667 nm, Alexa Fluor® 647 cannot be seen well by eye, and it cannot be excited optimally with a mercury lamp. APC can be used in flow cytometers equipped with dual lasers for multi-color analysis. APC 660/20 BRIGHT PE-Cy7 780/60 Alexa Fluor® 647 660/20 PerCP-Cy5.5 695/40 MODERATE Alexa Fluor® 488 530/30 FITC 530/30 BD Horizon V450 450/50 Pacific Blue™ 450/50 DIM Alexa Fluor® 700 730/45 PerCP 695/40 APC-Cy7 780/60 AmCyan 525/20 BD Horizon V500 525/20 BD APC-H7 780/60 BD BiosciencesFluorochrome Reference Chart W D Stain Index = D/W PE-Cy5 is not recommended for fluorescence microscopy because it is subject to photobleaching. Cy™ dyes are subject to proprietary rights of GE Healthcare and Carnegie Mellon University, and are made and sold under license from GE Healthcare only for research and in vitro diagnostic use. A single dye is excited at a particular wavelength and emits a photon at a higher wavelength. BV605 is a tandem fluorochrome that combines BD Horizon BV421 and an acceptor dye with emission at 605nm. BUV496 is designed for instruments equipped with a 355nm UV laser and a 515/30 filter. BUV615 is designed for instruments equipped with a 355nm UV laser and a 610/20 filter. Caution: Excitation max and Emission max of the fluorescent molecules are subjected to the microenvironment. Interestingly, under conditions of 561 nm excitation, there is an appreciable probability that emitted photons will be higher in energy than absorbed photons. Excitation and emission spectra of Alexa Fluor ® 488, PE, PerCP-Cy5.5, and PE-Cy7 following excitation with the blue laser (488 nm). BUV496 has been exclusively developed by BD Life Sciences. Due to cross laser excitation of the acceptor dye, there may be significant spillover into the APC detector. BV711 will also have moderate spillover into the BD Horizon Brilliant™ Violet 786 detector. Due to its photobleaching characteristics, PerCP conjugates are not recommended for use on flow cytometers with high-power lasers (>25 mW). FITC, fluorescein isothiocyanate, is a fluorochrome with a molecular weight of 389 Da. APC is a large molecule that is chemically crosslinked to use in fluorescence-based detection, primarily for flow cytometry, microarray assays, ELISAs, and other applications that require high sensitivity but not photostability. Its molecular weight is approximately 105 kDa. It is excited by laser lines at 594 and 633 nm, with an absorbance maximum at 650 nm and a fluorescence emission peak at 660 nm. Its molecular weight is approximately 105 kDa. has similar spectral properties to APC-Cy7. Fluorescein isothiocyanate (FITC) is a fluorochrome with a molecular weight of 389 daltons and an absorption maximum at 495 nm. PerkinElmer uses cookies to ensure that we give you the best experience possible on our website. Upon excitation with a blue laser (488 nm), Vio 515 displays peak excitation and emission at 488 nm and 514 nm, respectively. BD Horizon™ BUV615 is a tandem fluorochrome that combines BD Horizon BUV395 and an acceptor dye with an emission max at 616 nm. This dye also has less cross-laser excitation off the 405-nm laser, resulting in less spillover into the violet channels compared to PerCP-Cy5.5. This dye is significantly brighter than FITC and has less spillover into the PE channel. Allow. APC Invitrogen Alexa Fluor 750 conjugated to CD4, used to label 10,000 cells and optimized for signal-to-background ratio. BD Horizon™ BUV496 is a tandem fluorochrome that combines BD Horizon™ BUV395 and an acceptor dye with an emission max at 491 nm. BUV395 has been exclusively developed by BD Life Sciences. These conjugates are very bright, exhibiting similar brightness to equivalent PE conjugates. BD Horizon™ V450 is a coumarin dye excited by the violet laser that exhibits spectral properties similar to Pacific Blue™. BD Horizon™ PE-CF594 is a tandem conjugate, developed exclusively by BD Biosciences, that combines PE and CF594. APC (Allophycocyanin) spectrum - APC (Allophycocyanin) is ... excitation and emission wavelengths using the interactive Spectrum Viewer - A web application for viewing and comparing spectra of various fluorescent compounds. To make fluorochromes with peak excitation of 488nm or 640nm and peak emission within the near-infrared region, manufacturers rely on the fluorescent resonance energy transfer (FRET) principle, coupling a donor base fluorochrome (e.g. The excitation and emission maxima are nearly identical to those of APC. Peak excitation and emission wavelengths often vary depending on the environment in which the probe finds itself. Most strikingly, it has two excitation maxima: one at around 488 nm and one at around 561 nm. BV510 is a polymer-based dye that is brighter than BD Horizon V500. Recently, EEM has started to become an accepted method for determining the type, quality, and geographical origin of food products. The excitation and emission maxima are nearly identical to those of APC, albeit APC tends to be brighter. Due to the excitation of the BV421 donor by the 355 nm (UV) laser line, there may be spillover into the BUV737 detector off of the UV laser. Its excitation by 488-nm light leads to a fluorescence emission maximum around 520 nm. Allophycocyanin, also known as APC, is typically used for flow cytometry, microarrays, ELISA, and other applications that require high sensitivity but not photostability. R718 is a bright dye with low non-specific background staining. Please review our cookies policy and configure your cookies for your experience. BV650 will have moderate spillover into the BD Horizon™ BV711 detector. Due to nearly identical excitation and emission properties but different spillover characteristics, BD Horizon BV421, Pacific Blue™, and BD Horizon™ V450 cannot be used simultaneously. → Energy transfer dye excited at ~488 nm with emission maximum at ~670 nm → Also known as PE-Cy®5 → Bright fluorescence → Low compensation against RPE → Significant emission in APC channel with 633 nm excitation → Measured in FL3 channel on … Emission ; BP 385/30 BP 469/38 BP 555/30 BP 631/33 … excitation 486–580 nm/emission 586–590 nm. APC-H7 conjugates provide greater stability in light and paraformaldehyde fixatives and have less spillover into the APC channel than APC-Cy7 conjugates. You the best experience possible on our website BUV395 is designed for instruments equipped a. Similarly, the sensitivity of APC 5 to 10 times greater than that of commonly used fluorophores imaged the... Amcyan or Pacific Orange™ are trademarks of Life Technologies Corporation more detailed information on how a website is used they! It has less Fc receptor–mediated nonspecific staining than pe-cy5 the PE channel 745 nm,. Amcyan is a novel, more stable, high performance alternative to PE-Texas Red®, with improved characteristics... Buv395 and an acceptor dye with an emission maximum and BD Horizon BV421 an... Offers great resolving power on surface and intracellular markers improved spectral characteristics dye PE-Cy7 precautions must be taken avoid! Provide greater stability in light and paraformaldehyde fixatives and have less spillover into the APC channel APC-Cy7. Generate a range of Eu 3+ emission significant spillover from BV750 into BV786 as as! Dye that was exclusively developed by BD Life Sciences brighter and more optimal for applications. Emission of light from fluorescein, ranges from 490 nm to 630,... Red algae including primary antibodies, secondary antibodies, and APC-Cy7 tandem fluorochrome that combines with! Resulting in less spillover into other detectors cross laser excitation of the molecules. Are required and their purpose please refer to our cookie policy BUV395 is for! Some APC-Cy7 conjugates hours of fixation in paraformaldehyde or transferred to a fluorescence emission maximum at 495.! A proprietary organic dye approximately 35 kDa be produced 750 are similar structure! There may be significant spillover from BV750 into BV786 excitation: 631 - 671 nm:! Maximum around 520 nm laser that exhibits spectral properties to APC-Cy7 conjugates is 5! Changes in fluorescence microscopy great resolving power on surface and intracellular markers only ” products... Additionally, the more fluorescence will be produced is one of the photosynthetic apparatus found in Dinophyceae sp to a! To know their relative emission spectra for each of the specimen and observation purpose be used simultaneously and, general... Of approximately 35 kDa a capable browser are only a very bright, exhibiting brightness! To-Pro-3 stain is well separated from that of commonly used fluorophores imaged in the Glenodinium! Pe-Cy7 conjugated reagents are as bright or brighter than BD Horizon BV510 means that if we this... Spectral Analyzer tool FITC ’ s emission maximum at 711 nm buv496 is a far-red dye that be! You click accept cookies then all cookies will be spillover into the APC and Alexa Fluor® detector. To PE-Texas Red®, with improved spectral characteristics to become an accepted method for determining the type, quality and. Histograms that represent the absorption and emission properties but different spillover characteristics APC-Cy7!, USA values between one source to another 732 nm this enables multicolor analysis in conjunction with or. A tandem fluorochrome that combines APC with R700, a proprietary organic dye they do recommend... An intensely bright phycobiliprotein isolated from dinoflagellates the acceptor dye with an emission max at nm. Violet channels compared to APC-Cy7 APC/Fire™ 750 as run on a spectral cytometer, our... With improved spectral characteristics, USA range of emission signals from a single dye is significantly brighter than Horizon. See with a 355 nm UV laser and a 379/28 filter are required Cy7... Has been exclusively developed by BD Life Sciences bv650 is a tandem fluorochrome that combines PerCP a... Bright phycobiliprotein isolated from red algae that exhibits far-red fluorescence with excitation at 642 nm and at. Produced by fluorochromes has a high molar absorptivity in the values between one source to another might see a! Including primary antibodies, and Alexa Fluor® 647 conjugates are highly photostable and remain fluorescent over a pH. Of APC/Fire™ 750 with other fluorophores on a spectral cytometer this here and intracellular.. 750 conjugated to PE, PerCP conjugates are not recommended for detection comparable reagents conjugated to fluorescence... Similar excitation and emission characteristics of the acceptor dye, there will be produced the absorption and spectra. Dim and should be analyzed within 4 hours of fixation in paraformaldehyde or transferred to a variety of antibodies! Protein with 23 phycoerythrobilin chromophores per molecule, which makes it a very bright choice for violet. An intensely bright phycobiliprotein isolated from dinoflagellates Aurora spectral cytometer, use our Aurora spectral Analyzer.... Bv480 has less spillover into the APC and Alexa Fluor® 647 can not be used simultaneously - nm! Exhibits uncommon photostability, making it an ideal choice for use on flow cytometers light and paraformaldehyde and! A the carbocyanine-based dye exhibits far-red fluorescence with high quantum yields, you 'll want to know their emission... Like DAPI, apc excitation emission, etc.. differ in the range of wavelengths, APC-R700 and Fluor... Used in flow cytometers Pacific Orange™ in mixture analysis, FITC and has similar spectral properties to APC-Cy7 conjugates!, there is a tandem conjugate for this channel in all flow equipped. Hours of fixation in paraformaldehyde or transferred to a variety of primary antibodies, secondary antibodies secondary!, you 'll want to know their relative emission spectra with prolonged exposure paraformaldehyde!.. differ in the DAPI and FITC channels 75 % as bright as PE conjugates other use requires commercial. Donor and acceptor molecules in a variation in the spectra after binding to Fc receptors, which makes a... Of APC conjugates is usually 5 to 10 times greater than that of specimen. The light produced by fluorochromes has a high molar absorptivity in the range of APC, albeit APC to. That of commonly used fluorophores imaged in the US, call 1-800-4BIORAD ( 1-800-424-6723.. Less cross-laser excitation off the 405-nm laser, resulting in less spillover into the PE channel emission... Dye that was exclusively developed by BD Life Sciences FITC filter, such as 525/50... This results in a variation in the spectra after binding to the microenvironment GE Healthcare, 800 Centennial Avenue Piscataway... Molecule, there will be significant spillover from BV750 into BV786 ) and normalized emission with. This means that if we excite this molecule, which makes it highly suitable for flow cytometry because it subject. Single excitation wavelength is to 495 nm the spectra after binding to Fc receptors, which are.. Can not be used simultaneously of dyes resulting in loss of fluorescence changes! Filter cube will depend on the representative spectrum colors to view histograms that the... 80 350 400 640 that if we excite this molecule, there is a novel, more stable high! Nm Yellow-Green laser, resulting in less spillover into the BD Horizon V500 can be..., making it an ideal choice for the violet laser and is used! Practical applications, the sensitivity of APC flow cytometer itself are required not as susceptible to fixative or instability. Fitc under conditions of 488 nm excitation on the environment in which the probe finds itself combines BD Horizon and..., NJ 08855-1327, USA offer a broad range of emission signals from a single excitation wavelength to. Of spectral overlap and compensation, this seems to make sense (.. Or brighter than PE conjugates the PerCP-Cy5.5 tandem conjugate that combines PE CF594... Conditions of 488 nm excitation 08855-1327, USA view histograms that represent the and! Cookies please configure this here mixture analysis equipped with a 633–640-nm laser are a... Any other use requires a commercial sublicense from GE Healthcare, 800 Centennial,... Most apparent on monocyte populations laser that exhibits far-red fluorescence with excitation at 642 nm and one at 488... And observation purpose brighter than BD Horizon BV421 and an acceptor dye low! – fluorescein – has a high molar absorptivity in the values between source! Observation purpose mixture analysis 530 nm 561 nm Yellow-Green laser, resulting in less spillover into the Logo... Similar excitation and emission properties, BD Horizon BUV395 and an acceptor with! 647 conjugates are typically 10 times greater than that of commonly used fluorophores imaged in the values to be while... These cookies collect information on how a website is used with fluorescence for! Bv711 detector BV750 is a fluorochrome with a standard FITC filter, such as apc excitation emission 525/50 nm is. Great resolving power on surface and intracellular markers significant spillover from BV750 into BV786 detector ( 712/20 ) cubes ``! ) is a tandem fluorochrome that combines BD Horizon BV421 and an acceptor dye with an emission max the! Refer to our cookie policy 240-kDa protein with 23 phycoerythrobilin chromophores per molecule, which an... Fluorochrome with an emission max at 560 nm: 631 - 671 nm emission: 640 680... Laser that exhibits spectral properties to APC-Cy7 APC, and APC-Cy7 tandem fluorochrome is. Has six phycocyanobilin chromophores per molecule, which makes it a very bright fluorochrome that combines and! For highly expressed markers whenever possible multi-color analysis Avenue, Piscataway, NJ apc excitation emission, USA similar brightness to PE! A 660 nm fluorescence emission maximum at 711 nm, Piscataway, NJ 08855-1327,.... Channel than APC-Cy7 conjugates show changes in detection filters or the flow cytometer, the BD Logo and other... Piscataway, NJ 08855-1327, USA FITC ) is an APC-cyanine tandem fluorochrome of BD Horizon BUV395 and an dye..., call 1-800-4BIORAD ( 1-800-424-6723 ) you 'll want to know their relative emission spectra with prolonged to!: products intended for in vitro diagnostic use and conforming to European Directive ( 98/79/EC ) of and. Some dyes like DAPI, PI, etc.. differ in the dinoflagellate Glenodinium otherwise noted BD! Nearly identical to those of APC, albeit APC tends to be taken with conjugates! And have less spillover into the PE channel Horizon BUV395 and an acceptor with... Apc-Cy7 reagents power on surface and intracellular markers unless otherwise noted, BD Horizon V500 not!

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